LOCUS       40924_41052        3369 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSpark V, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Paz E.
  TITLE     pSpark V DNA Cloning Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Paz E.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2009) Molecular Biology, Canvax Biotech S.L., 
            Estocolmo, Cordoba, Cordoba 14014, Spain
REFERENCE   3  (bases 1 to 3369)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-AUG-2009) Molecular Biology, Canvax Biotech S.L., Estocolmo, 
            Cordoba, Cordoba 14014, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             109..297
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    402..546
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(732..1320)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1494..2351)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2352..2456)
                     /label=AmpR promoter
     rep_origin      complement(2534..2989)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3130..3146
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3153..3171
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3307..3325)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3343..3359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accggccggt catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
       61 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
      121 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
      181 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
      241 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
      301 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
      361 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
      421 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
      481 gagtgcacca tatatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
      541 atcaggcgct ctaccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      601 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      661 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      721 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      781 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      841 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      901 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      961 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1021 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1081 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1141 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     1201 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1261 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1321 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1381 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1441 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1501 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1561 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1621 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1681 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1741 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1801 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1861 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1921 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1981 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     2041 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     2101 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     2161 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     2221 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     2281 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2341 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2401 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2461 tttccccgaa aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2521 ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     2581 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2641 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2701 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2761 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2821 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2881 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2941 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat
     3001 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     3061 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     3121 cacgacgttg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg
     3181 gcccccatgg gtttaaacga attctccgga tcaggcctga tatcaggatc cactagtgcg
     3241 gccgcctgca ggtcgacttt catatgtttg agctcgagta acgcgttgga tgcatagctt
     3301 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgc
     3361 tcttccgct
//
